Journal: iScience

Article Title: SARS-CoV-2 infects an in vitro model of the human developing pancreas through endocytosis

doi: 10.1016/j.isci.2022.104594

Figure Lengend Snippet:

Article Snippet: Mouse anti-TMPRSS2 , DSHB , Cat# P5H9-A3; RRID: AB_2205599.

Techniques: Control, Virus, Recombinant, Reverse Transcription, Luminex, Software

Journal: Scientific Reports

Article Title: Distinct effects of β1 integrin on cell proliferation and cellular signaling in MDA-MB-231 breast cancer cells

doi: 10.1038/srep18430

Figure Lengend Snippet: ( A ) WT and KO cells were immunoblotted by anti-β1 antibody, α-tubulin was used as a loading control (left panel). WT and KO cells were collected and incubated with (bold line) or without (grey shadow) anti-β1, followed by incubation with Alexa Fluor 647 goat anti-mouse IgG subjected to flow cytometry analysis (right panel). ( B ) Cell lysates from WT and KO cells were immunoblotted with anti-α3, anti-α5, anti-α6, anti-αV and anti-β4 antibodies. α-Tubulin was used as a loading control (upper panel). Cells were collected and incubated with (bold line) or without (grey shadow) anti-α3, anti-α5, anti-α6 and anti-β4 antibodies, followed by incubation with Alexa Fluor 647 goat anti-mouse IgG subjected to flow cytometry analysis (lower panel). ( C ) Bright field pictures were taken to show the representative cell morphology. Scale bar, 50 μm (upper panel). WT and KO cells were stained with anti-β1 antibody, followed by incubation with fluorescent secondary antibody. Localization of F-actin was examined by staining with Alexa Fluor 546 phalloidin, the bar denotes 20 μm (middle and low panel). ( D ) The indicated cells were stained with anti-pFAK and anti-β4 antibody, followed by the incubation with fluorescent secondary antibody. Scale bar, 20 μm. The arrows indicate β4 integrin, the hemidesmosome maker, expressed in the cell-cell contact.

Article Snippet: The experiments were performed using the following antibodies: Antibody against human integrin β1 subunit (P5D2) was from Developmental Studies Hybridoma Bank, University of Iowa; Mouse mAb against Smad2, rabbit mAbs against EGFR, p-EGFR, ERK1/2, p-ERK1/2, AKT, p-AKT, p-Src, and p-Smad2 were from Cell Signaling Technology; rabbit pAb to β4 integrin and goat antibody against α3 integrin were from Santa Cruz Biotechnology (Santa Cruz, CA); mouse mAbs against β1 integrin, α5 integrin, β4 integrin, αv integrin, FAK, p-FAK, and rat antibody against α6 integrin were from BD Biosciences; mAb against α-tubulin and α-SMA were from Sigma; mouse mAbs against Src was from upstate biotechnology.

Techniques: Control, Incubation, Flow Cytometry, Staining

Journal: Scientific Reports

Article Title: Distinct effects of β1 integrin on cell proliferation and cellular signaling in MDA-MB-231 breast cancer cells

doi: 10.1038/srep18430

Figure Lengend Snippet: ( A ) After attachment for 24 h, WT and KO cells treated with 3 μM of AG1478 for 48 h, the number of live cells were counted. Cell numbers were normalized to those at 0 h. Data are represented as the means ± s.e.m (*** p < 0.001 by two-tail unpaired t-test). ( B ) MDA-MB-231 cells were untreated, or treated with P5D2, AG1478, or P5D2 plus AG1478 for 48 h. Cell proliferation was evaluated by the number of live cells. Cell numbers were normalized to those at 0 h as 1. Data are represented as the means ± s.e.m (** p < 0.01 by two-tail unpaired t-test).

Article Snippet: The experiments were performed using the following antibodies: Antibody against human integrin β1 subunit (P5D2) was from Developmental Studies Hybridoma Bank, University of Iowa; Mouse mAb against Smad2, rabbit mAbs against EGFR, p-EGFR, ERK1/2, p-ERK1/2, AKT, p-AKT, p-Src, and p-Smad2 were from Cell Signaling Technology; rabbit pAb to β4 integrin and goat antibody against α3 integrin were from Santa Cruz Biotechnology (Santa Cruz, CA); mouse mAbs against β1 integrin, α5 integrin, β4 integrin, αv integrin, FAK, p-FAK, and rat antibody against α6 integrin were from BD Biosciences; mAb against α-tubulin and α-SMA were from Sigma; mouse mAbs against Src was from upstate biotechnology.

Techniques:

Journal: Respiratory Research

Article Title: FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis

doi: 10.1186/s12931-018-0768-1

Figure Lengend Snippet: Primer table for Real-Time Quantitative Reverse-Transcriptase PCR (qRT-PCR). Primers were synthesized by MWG Eurofins (Ebersberg, Germany).

Article Snippet: Integrin-β1 , ITGB1 , mouse monoclonal anti-ITGB1 antibody , Abcam, Cambridge, UK , WB.

Techniques: Synthesized

Journal: Respiratory Research

Article Title: FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis

doi: 10.1186/s12931-018-0768-1

Figure Lengend Snippet: Primary antibodies used in Western Blot analysis, Immunofluorescence and Proximity Ligation Assays

Article Snippet: Integrin-β1 , ITGB1 , mouse monoclonal anti-ITGB1 antibody , Abcam, Cambridge, UK , WB.

Techniques: Western Blot, Immunofluorescence, Ligation

Journal: Respiratory Research

Article Title: FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis

doi: 10.1186/s12931-018-0768-1

Figure Lengend Snippet: FKBP10 deficiency alters the expression of molecules implicated in adhesion and migration. a, c, e, g, i Western Blot analysis of phLF treated with scrambled siRNA as control (sc) or FKBP10 siRNA (kd) and 2- phosphoascorbate (0.1 mM) in absence and presence of TGF-β1 (2 ng/mL) for 48h. Densitometric analysis and representative blots show the effect of FKBP10 kd on the expression of talin-1 (TLN1) ( a ), calpain-4 (CAPNS1) ( c ), integrin β1 (ITGB1) ( e ), caveolin-1 (CAV1) ( g ) and coronin 1C (CORO1C) ( i ) relative to β-actin as loading control (ACTB). b, d, f, h, j Quantitative reverse transcriptase-polymerase chain reaction analysis of phLF treated with scrambled siRNA as control (sc) or FKBP10 siRNA (kd) and 2- phosphoascorbate (0.1 mM) in absence and presence of TGF-β1 (2 ng/mL) for 48h. Transcript levels are presented as -ΔCt values showing the effect of siRNA mediated kd of FKBP10 on talin-1 (TLN1) ( b ), calpain-4 (CAPNS1) ( d ), integrin β1 (ITGB1) ( f ), caveolin-1 (CAV1) ( h ) and coronin 1C (CORO1C) ( j ). DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 (DHX8) was used as endogenous control. All data is based on eight (protein) or seven (mRNA) independent experiments. Statistical significance between control and FKBP10 kd is indicated by horizontal brackets and asterisks

Article Snippet: Integrin-β1 , ITGB1 , mouse monoclonal anti-ITGB1 antibody , Abcam, Cambridge, UK , WB.

Techniques: Expressing, Migration, Western Blot, Polymerase Chain Reaction

Journal: Cell and Tissue Banking

Article Title: Multilineage potential research of Beijing duck amniotic mesenchymal stem cells

doi: 10.1007/s10561-018-9701-6

Figure Lengend Snippet: Primer sequences used for RT-PCR

Article Snippet: The direct antibodies used were FITC goat anti-rabbit CD29 (1:100; BIOSS), FITC goat anti-rabbit CD166 (1:100; BIOSS), FITC goat anti-rabbit CD71 (1:100; BIOSS), FITC goat anti-rabbit CD105 (1:100; BIOSS) and FITC goat anti-rabbit OCT4 (1:100; BIOSS).

Techniques:

Journal: Cell and Tissue Banking

Article Title: Multilineage potential research of Beijing duck amniotic mesenchymal stem cells

doi: 10.1007/s10561-018-9701-6

Figure Lengend Snippet: Detection of surface markers in AMSCs. a The AMSCs could express pluripotent marker gene OCT4 and MSC-associated markers by immunofluorescence stain (bar = 50 μm), DAPI, Blue. b mRNA expression levels of AMSCs markers were detected by RT-PCR, but the expression of CD34 and CD45 were not detected. c AMSCs at P4 were colabeled with surface antigens (CD29, CD166, CD71, CD105, OCT4), and the positive rates were all above 99% by flow cytometry analysis

Article Snippet: The direct antibodies used were FITC goat anti-rabbit CD29 (1:100; BIOSS), FITC goat anti-rabbit CD166 (1:100; BIOSS), FITC goat anti-rabbit CD71 (1:100; BIOSS), FITC goat anti-rabbit CD105 (1:100; BIOSS) and FITC goat anti-rabbit OCT4 (1:100; BIOSS).

Techniques: Marker, Immunofluorescence, Staining, Expressing, Reverse Transcription Polymerase Chain Reaction, Flow Cytometry